Aliidiomarina quisquiliarum sp. nov., isolated from landfill leachate

Bacterial strain Y-6^T, isolated from a landfill site in Yiwu, PR China, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed pale beige colonies. Strain Y-6^T grew at 4-40 °C (optimal at 30-37 °C), pH 6.5-9.5 (optimal at pH 7.2-8.5) and in the presence of 0.5-10.0 % (w/v) NaCl (optimal at 1.0-3.0 %). Phylogenetic analysis revealed that strain Y-6^T was a member of the genus Aliidiomarina and closely related to Aliidiomarina taiwanensis MCCC 1A06493^T with a 16S rRNA sequence similarity of 98.2 %. The major cellular fatty acids of the isolate were iso-C_{15 : 0}, C_{16 : 0}, iso-C_{17 : 0} and summed feature 9 (iso-C_{17 : 1} ω9c and/or 10-methyl-C_{16 : 0}). Q-8 was the predominant ubiquinone. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycophospholipid, aminophospholipid, phospholipid, three glycolipids and two unknown lipids. The genomic DNA G+C content was 46.6 mol%. The digital DNA-DNA hybridization value between Y-6^T and A. taiwanensis MCCC 1A06493^T was 18.3 %. Strain Y-6^T had an average nucleotide identity value of 74.09 % with A. taiwanensis MCCC 1A06493^T. Results from the polyphasic taxonomy study support the conclusion that strain Y-6^T represents a novel Aliidiomarina species, for which the name Aliidiomarina quisquiliarum sp.nov. is proposed. The type strain is Y-6^T (=MCCC 1K06228^T=KCTC 82676^T).