Mol Cell Endocrinol. 1987 Aug;52(3):267-72.

Sequencing of cDNA inserts encoding aromatase cytochrome P-450 (P-450AROM).

Simpson ER, Evans CT, Corbin CJ, Powell FE, Ledesma DB, Mendelson CR.

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Department of Obstetrics-Gynecology, University of Texas Health Science Center, Dallas 75235-9051.

Abstract

Two cDNA inserts complementary to mRNA encoding aromatase cytochrome P-450 (P-450AROM) have been isolated and characterized by restriction mapping and sequencing. The overlapping sequence encoded by these inserts is identical, and a putative heme-binding region has been identified. In addition, the open reading frame contains the sequences of all four cysteine-containing tryptic peptides isolated by Chen et al. (1986) from purified cytochrome P-450AROM. The inserts differ in the use of alternative poly A-addition signals, which is consistent with the presence of two major species of mRNA in human placenta, of 3.0 and 2.4 kb, which hybridize to these inserts. The identity of sequence between the two inserts and the likely presence of alternative poly A-addition signals, is suggestive that only one form of cytochrome P-450AROM is encoded by these mRNA species.

PMID:: 3653507; [PubMed - indexed for MEDLINE]

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Alternative usage of different poly(A) addition signals for two major species of mRNA encoding human aromatase P-450. [FEBS Lett. 1989]
Alternative usage of different poly(A) addition signals for two major species of mRNA encoding human aromatase P-450.
Toda K, Terashima M, Mitsuuchi Y, Yamasaki Y, Yokoyama Y, Nojima S, Ushiro H, Maeda T, Yamamoto Y, Sagara Y. FEBS Lett. 1989 Apr 24; 247(2):371-6.
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Isolation and characterization of a complementary DNA specific for human aromatase-system cytochrome P-450 mRNA. [Proc Natl Acad Sci U S A. 1986]
Isolation and characterization of a complementary DNA specific for human aromatase-system cytochrome P-450 mRNA.
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Cited by 3 PubMed Central articles

Proteins from eight eukaryotic cytochrome P-450 families share a segmented region of sequence similarity. [Proc Natl Acad Sci U S A. 1988]
Proteins from eight eukaryotic cytochrome P-450 families share a segmented region of sequence similarity.
Kalb VF, Loper JC. Proc Natl Acad Sci U S A. 1988 Oct; 85(19):7221-5.
Isolation of a full-length cDNA insert encoding human aromatase system cytochrome P-450 and its expression in nonsteroidogenic cells. [Proc Natl Acad Sci U S A. 1988]
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Corbin CJ, Graham-Lorence S, McPhaul M, Mason JI, Mendelson CR, Simpson ER. Proc Natl Acad Sci U S A. 1988 Dec; 85(23):8948-52.
Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450. [EMBO J. 1989]
Phosphorylation of hepatic phenobarbital-inducible cytochrome P-450.
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