A novel c-type lysozyme from Litopenaeus vannamei exhibits potent antimicrobial activity

Feng Hu; Yan Wang; Jingjie Hu; Zhenmin Bao; Mengqiang Wang

doi:10.1016/j.fsi.2022.10.056

A novel c-type lysozyme from Litopenaeus vannamei exhibits potent antimicrobial activity

Fish Shellfish Immunol. 2022 Dec:131:729-735. doi: 10.1016/j.fsi.2022.10.056. Epub 2022 Oct 29.

Authors

Feng Hu¹, Yan Wang², Jingjie Hu³, Zhenmin Bao³, Mengqiang Wang⁴

Affiliations

¹ MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China.
² MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China.
³ MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China; Laboratory for Marine Fisheries Science and Food Production Processes, Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China.
⁴ MOE Key Laboratory of Marine Genetics and Breeding (Qingdao 266003), and Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institute (Sanya 572024), Ocean University of China, China; Laboratory for Marine Fisheries Science and Food Production Processes, Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China. Electronic address: wangmengqiang@ouc.edu.cn.

PMID: 36341874
DOI: 10.1016/j.fsi.2022.10.056

Abstract

Lysozyme is relevant to the innate immune system as a vital protein for crustaceans. In the present study, we cloned and characterized a novel c-type lysozyme gene (LvLYZ) from the Pacific white shrimp (Litopenaeus vannamei). The obtained full-length cDNA of LvLYZ was 990 bp and contained an open reading frame of 693 bp. Its deduced amino acid sequence consisted of 230 amino acids (aa) with a 17 aa signal peptide at the N-terminal and 130 aa functional domains. The multiple sequence alignment (MSA) indicated that the typical active sites in LvLYZ were similarly conserved as c-type lysozymes from other species. The transcription of LvLYZ appeared in all detected tissues and had relatively higher expression levels in hemocytes, hepatopancreas, gill and intestine. The mRNA expression profiles of LvLYZ were up-regulated in hemocyte and hepatopancreas post the stimulation of Vibrio parahaemolyticus or white spot syndrome virus (WSSV), respectively. The recombinant protein of LvLYZ (rLvLYZ) exhibited antibacterial activities against various microbes, including Escherichia coli, Vibrio splendidus, Micrococcaus luteus, Vibrio parahaemolyticus and Staphylococcus aureus. These results indicated that LvLYZ could cope with bacteria in L. vannamei and may play a significant role in immune response against invading pathogens.

Keywords: Antimicrobial activity; Litopenaeus vannamei; Novel lysozyme.

MeSH terms

Animals
Anti-Infective Agents* / pharmacology
Arthropod Proteins
Base Sequence
Immunity, Innate / genetics
Muramidase / genetics
Muramidase / metabolism
Penaeidae*
Phylogeny
Vibrio parahaemolyticus* / physiology
White spot syndrome virus 1* / physiology

Substances

Muramidase
Arthropod Proteins
Anti-Infective Agents