423 Vibrio cholerae non-group O-1 strains were tested for vibriocin production using a homologous set of 7 indicator strains. Using this scheme, 334 (79.4%) strains proved to be typable. The strains isolated from 7 different geographical areas of Romania exhibited 16 different patterns of vibriocin production. The presence of a predominant pattern per year and per region was revealed.