Chryseobacterium oryzae sp. nov. and Chryseobacterium suipulveris sp. nov., isolated from Andong sikhye and pigpen dust, respectively

Two Gram-stain-negative, aerobic, mesophilic, rod-shaped bacteria, ADR-1^T and SC2-2^T, were isolated from Andong sikhye and dust in a pigpen, respectively. The phylogenetic tree on the basis of 16S rRNA gene sequences showed that strains ADR-1^T and SC2-2^T were members of the genus Chryseobacterium and revealed the highest sequence similarities to Chryseobacterium binzhouense LM2^T (97.6 %) and Chryseobacterium koreense Chj707^T (94.9 %), respectively. Phylogenomic treeing using 92 core genes clearly indicated that strain ADR-1^T clustered with Chryseobacterium echinoideorum CC-CZW010^T, Chryseobacterium binzhouense LM2^T and Chryseobacterium taihuense CGMCC 1.10941^T, and strain SC2-2^T formed a compact cluster with Chryseobacterium koreense CCUG 49689^T. The digital DNA-DNA hybridization (dDDH) and orthologous average nucleotide identity (ANI) values of strain ADR-1^T with the closely related species of the genus Chryseobacterium were ≤24.4 % and ≤80.7 %, and those of strain SC2-2^T were ≤24.0 % and ≤77.8 %, respectively, which are well below the cut-off values of species discrimination (>70 % dDDH and >95-96 % ANI). The only respiratory quinone in both strains was menaquinone 6. The polar lipid profile of strain ADR-1^T comprised phosphatidylethanolamine, four unidentified aminolipids and three unidentified lipids, while strain SC2-2^T contained phosphatidylethanolamine, two unidentified aminolipids, one unidentified aminophospholipid and five unidentified polar lipids. The major fatty acids (>10 %) of strain ADR-1^T were iso-C_{15 : 0}, summed feature 9 (iso-C_{17 : 1} ω9c and/or C_{16 : 0} 10-methyl), iso-C_{17 : 0} 3-OH and summed feature 3 (C_{16 : 1} ω7c and/or C_{16 : 1} ω6c), and those of strain SC2-2^T were iso-C_{15 : 0} and anteiso-C_{15 : 0}. On the basis of the results of phenotypic and phylogenetic analyses, strains ADR-1^T and SC2-2^T represent two distinct novel species in the genus Chryseobacterium, for which the names Chryseobacterium oryzae sp. nov. (type strain ADR-1^T=KACC 19311^T=NBRC 113104^T) and Chryseobacterium suipulveris sp. nov. (type strain SC2-2^T=KACC 19313^T=NBRC 113106^T) are proposed.