Ozone Impact on Emission of Biogenic Volatile Organic Compounds in Three Tropical Tree Species From the Atlantic Forest Remnants in Southeast Brazil

Plants emit a broad number of Biogenic Volatile Organic Compounds (BVOCs) that can impact urban ozone (O₃) production. Conversely, the O₃ is a phytotoxic pollutant that causes unknown alterations in BVOC emissions from native plants. In this sense, here, we characterized the constitutive and O₃-induced BVOCs for two (2dO₃) and four (4dO₃) days of exposure (O₃ dose 80 ppb) and evaluated the O₃ response by histochemical techniques to detect programmed cell death (PCD) and hydrogen peroxide (H₂O₂) in three Brazilian native species. Croton floribundus Spreng, Astronium graveolens Jacq, and Piptadenia gonoacantha (Mart.) JF Macbr, from different groups of ecological succession (acquisitive and conservative), different carbon-saving defense strategies, and specific BVOC emissions. The three species emitted a very diverse BVOC composition: monoterpenes (MON), sesquiterpenes (SEQ), green leaf volatiles (GLV), and other compounds (OTC). C. floribundus is more acquisitive than A. graveolens. Their most representative BVOCs were methyl salicylate-MeSA (OTC), (Z) 3-hexenal, and (E)-2-hexenal (GLV), γ-elemene and (-)-β-bourbonene (SEQ) β-phellandrene and D-limonene (MON), while in A. graveolens were nonanal and decanal (OTC), and α-pinene (MON). Piptadenia gonoachanta is more conservative, and the BVOC blend was limited to MeSA (OTC), (E)-2-hexenal (GLV), and β-Phellandrene (MON). The O₃ affected BVOCs and histochemical traits of the three species in different ways. Croton floribundus was the most O₃ tolerant species and considered as an SEQ emitter. It efficiently reacted to O₃ stress after 2dO_3, verified by a high alteration of BVOC emission, the emergence of the compounds such as α-Ionone and trans-ß-Ionone, and the absence of H₂O₂ detection. On the contrary, A. graveolens, a MON-emitter, was affected by 2dO₃ and 4dO₃, showing increasing emissions of α-pinene and β-myrcene, (MON), γ-muurolene and β-cadinene (SEQ) and H₂O₂ accumulation. Piptadenia gonoachanta was the most sensitive and did not respond to BVOCs emission, but PCD and H₂O₂ were highly evidenced. Our results indicate that the BVOC blend emission, combined with histochemical observations, is a powerful tool to confirm the species' tolerance to O₃. Furthermore, our findings suggest that BVOC emission is a trade-off associated with different resource strategies of species indicated by the changes in the quality and quantity of BVOC emission for each species.