Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Mukhayyo Sultonova; Devanand M Pinto; J Patrick Murphy

doi:10.1007/978-1-0716-2376-3_16

Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags

Methods Mol Biol. 2022:2508:211-223. doi: 10.1007/978-1-0716-2376-3_16.

Authors

Mukhayyo Sultonova¹, Devanand M Pinto², J Patrick Murphy³

Affiliations

¹ Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada.
² National Research Council, Halifax, NS, Canada.
³ Department of Biology, University of Prince Edward Island, Charlottetown, PE, Canada. jpmurphy@upei.ca.

PMID: 35737243
DOI: 10.1007/978-1-0716-2376-3_16

Abstract

Comparing cancer proteomes across many samples offers a window into cancer cell biology and may reveal new treatment options for specific subsets of cancer. Here we describe a method using tandem mass tag (TMT) technology to multiplex up to 18 samples in a single analysis, paving the way for the analysis of large cohorts of tumors, cell lines, and perturbations thereof. The procedure we describe will result in samples ready for in-depth LC-MS/MS analysis in 3-4 days.

Keywords: Isobaric labeling; LC-MS/MS; Proteomic profiling; SP3.

MeSH terms

Chromatography, Liquid / methods
Neoplasms*
Proteome / analysis
Proteomics* / methods
Tandem Mass Spectrometry / methods

Substances

Proteome