Eur J Biochem. 1987 Apr 15;164(2):309-15.

Purification and characterization of a ribonuclease specific for poly(U) and poly(C) from the larvae of Ceratitis capitata.

Abstract

A specific ribonuclease was detected and purified to homogeneity from six-day-old larvae of the insect Ceratitis capitata and its homogeneity was checked by analysis in polyacrylamide gels in the presence of sodium dodecyl sulfate. The nuclease specifically degrades poly(U) and poly(C) whilst it fails to do so with other single-stranded homopolyribonucleotides. The enzyme has a pH optimum in the region 7-9 and relative molecular mass of about 25,000. The effect of this ribonuclease on the integrity of RNAs isolated from six-day-old larvae or rat liver was also studied.

PMID:: 3569265; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Ribosome-associated ribonucleases from six-day larvae of the insect Ceratitis capitata. [Biochem Mol Biol Int. 1995]
Ribosome-associated ribonucleases from six-day larvae of the insect Ceratitis capitata.
Kouyanou S, Pilali M, Fragoulis EG. Biochem Mol Biol Int. 1995 Dec; 37(6):1217-27.
Preparation of monoclonal antibodies against a poly(U), poly(C) specific ribonuclease prepared from the insect Ceratitis capitata. [Biochem Int. 1992]
Preparation of monoclonal antibodies against a poly(U), poly(C) specific ribonuclease prepared from the insect Ceratitis capitata.
Sideris DC, Kühn S, Fragoulis EG. Biochem Int. 1992 Jun; 27(1):25-35.
Purification and characterization of a novel poly(U), poly(C) ribonuclease from Saccharomyces cerevisiae. [Biochim Biophys Acta. 1997]
Purification and characterization of a novel poly(U), poly(C) ribonuclease from Saccharomyces cerevisiae.
Lalioti VS, Ballesta JP, Fragoulis EG. Biochim Biophys Acta. 1997 Sep 26; 1342(1):62-72.
Isolation and characterization of the novel polyadenylate- and polyuridylate-degrading acid endoribonuclease V from calf thymus. [J Biol Chem. 1980]
Isolation and characterization of the novel polyadenylate- and polyuridylate-degrading acid endoribonuclease V from calf thymus.
Schröder HC, Dose K, Zahn RK, Müller WE. J Biol Chem. 1980 Jun 10; 255(11):5108-12.
In vitro translation of an intact mRNA coding for a poly(U), poly(C) specific ribonuclease isolated from six-day-old larvae of Ceratitis capitata by a modified extraction procedure. [Biochem Mol Biol Int. 1994]
In vitro translation of an intact mRNA coding for a poly(U), poly(C) specific ribonuclease isolated from six-day-old larvae of Ceratitis capitata by a modified extraction procedure.
Lalioti VS, Fragoulis EG. Biochem Mol Biol Int. 1994 Aug; 34(1):37-45.

See reviews... See all...

Cited by 2 PubMed Central articles

Cc RNase: the Ceratitis capitata ortholog of a novel highly conserved protein family in metazoans. [Nucleic Acids Res. 2003]
Cc RNase: the Ceratitis capitata ortholog of a novel highly conserved protein family in metazoans.
Rampias TN, Sideris DC, Fragoulis EG. Nucleic Acids Res. 2003 Jun 15; 31(12):3092-100.
A pyrimidine-guanine sequence-specific ribonuclease from Rana catesbeiana (bullfrog) oocytes. [Nucleic Acids Res. 1992]
A pyrimidine-guanine sequence-specific ribonuclease from Rana catesbeiana (bullfrog) oocytes.
Liao YD. Nucleic Acids Res. 1992 Mar 25; 20(6):1371-7.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound
PubChem chemical compound records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records. Multiple substance records may contribute to the PubChem compound record.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance
PubChem chemical substance records that cite the current articles. These references are taken from those provided on submitted PubChem chemical substance records.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Purification and characterization of a ribonuclease specific for poly(U) and pol...
Purification and characterization of a ribonuclease specific for poly(U) and poly(C) from the larvae of Ceratitis capitata.
Eur J Biochem. 1987 Apr 15 ;164(2):309-15.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: