The present study was conducted to evaluate the effects of dietary nano-nutrients on the growth, physiological and amino acid responses in tilapia fish. Vitamins were incorporated with chemically synthesized nanoparticles (Fe, Zn, Cu and Se) to form a nano-nutrient complex (NNC). Powder X-ray diffraction (PXRD) and scanning electron microscopy (SEM) analyses were performed to confirm the structure and morphology of the as-prepared nutrients. A commercial basal diet without the addition of any NNC was used as a control and compared with the other two diets formulated with different levels of NNC. In a 60 day feeding trial, the fish fed with a diet of NNC60 showed significant differences in final weight and length compared with the basal diet. Furthermore, a high value of nutrient content was observed in the muscles of fish fed with nano diets. In addition, protein, total fat, vitamin C, and essential amino acid levels were significantly higher in the NNC60-treated fish compared with the other groups. The present study suggests that the addition of NNC to a commercial diet has the potential to enhance the growth performance and biochemical parameters in tilapia fish.
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