Biochem J. 1986 Sep 1;238(2):475-83.

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.

Duncan K, Chaudhuri S, Campbell MS, Coggins JR.

Abstract

The enzyme 3-dehydroquinase was purified in milligram quantities from an overproducing strain of Escherichia coli. The amino acid sequence was deduced from the nucleotide sequence of the aroD gene and confirmed by determining the amino acid composition of the overproduced enzyme and its N-terminal amino acid sequence. The complete polypeptide chain consists of 240 amino acid residues and has a calculated subunit Mr of 26,377. Transcript mapping revealed that aroD is a typical monocistronic gene.

PMID:: 3541912; [PubMed - indexed for MEDLINE]
PMCID:: PMC1147159

Free PMC Article

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Secondary Source ID

GENBANK/X04306

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa. [Biochem J. 1988]
The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.
White PJ, Millar G, Coggins JR. Biochem J. 1988 Apr 15; 251(2):313-22.
Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi. [J Gen Microbiol. 1991]
Molecular cloning and characterization of the aroD gene encoding 3-dehydroquinase from Salmonella typhi.
Servos S, Chatfield S, Hone D, Levine M, Dimitriadis G, Pickard D, Dougan G, Fairweather N, Charles I. J Gen Microbiol. 1991 Jan; 137(1):147-52.
Cloning of dapD, aroD and asd of Leptospira interrogans serovar icterohaemorrhagiae, and nucleotide sequence of the asd gene. [J Gen Microbiol. 1992]
Cloning of dapD, aroD and asd of Leptospira interrogans serovar icterohaemorrhagiae, and nucleotide sequence of the asd gene.
Baril C, Richaud C, Fournié E, Baranton G, Saint Girons I. J Gen Microbiol. 1992 Jan; 138(1):47-53.
Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase. [Biochem J. 1988]
Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.
Anton IA, Coggins JR. Biochem J. 1988 Jan 15; 249(2):319-26.
The characterisation of the shikimate pathway enzyme dehydroquinase from Pisum sativum. [FEBS Lett. 1994]
The characterisation of the shikimate pathway enzyme dehydroquinase from Pisum sativum.
Deka RK, Anton IA, Dunbar B, Coggins JR. FEBS Lett. 1994 Aug 8; 349(3):397-402.

See reviews... See all...

Cited by 19 PubMed Central articles

Modular engineering of L-tyrosine production in Escherichia coli. [Appl Environ Microbiol. 2012]
Modular engineering of L-tyrosine production in Escherichia coli.
Juminaga D, Baidoo EE, Redding-Johanson AM, Batth TS, Burd H, Mukhopadhyay A, Petzold CJ, Keasling JD. Appl Environ Microbiol. 2012 Jan; 78(1):89-98. Epub 2011 Oct 21.
aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity. [J Bacteriol. 2008]
aro mutations in Salmonella enterica cause defects in cell wall and outer membrane integrity.
Sebkova A, Karasova D, Crhanova M, Budinska E, Rychlik I. J Bacteriol. 2008 May; 190(9):3155-60. Epub 2008 Feb 29.
Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae. [J Bacteriol. 2005]
Role of the Rep helicase gene in homologous recombination in Neisseria gonorrhoeae.
Kline KA, Seifert HS. J Bacteriol. 2005 Apr; 187(8):2903-7.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

The overexpression and complete amino acid sequence of Escherichia coli 3-dehydr...
The overexpression and complete amino acid sequence of Escherichia coli 3-dehydroquinase.
Biochem J. 1986 Sep 1 ;238(2):475-83.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: