Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
EMBO J. 1986 Nov;5(11):2995-3000.

Functional dissection of Escherichia coli promoters: information in the transcribed region is involved in late steps of the overall process.

Abstract

After binding to a promoter Escherichia coli RNA polymerase is in contact with a region of about 70 bp. Around 20 bp of this sequence are transcribed. Information encoded within this transcribed region is involved in late steps of the functional program of a promoter. By changing such 'down-stream' sequences promoter strength in vivo can be varied more than 10-fold. By contrast, information for early steps of the promoter program such as recognition by the enzyme and formation of a stable complex resides in a central core region of about 35 bp. Our data show that the strength of a promoter can be limited at different levels of the overall process. Consequently promoters of identical strength can exhibit different structures due to an alternate optimization of their program.

PMID:
3539590
[PubMed - indexed for MEDLINE]
PMCID:
PMC1167252
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for PubMed Central
    Loading ...
    Write to the Help Desk