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    Biochemistry. 1986 Jul 29;25(15):4366-71.

    Purification and characterization of aminoimidazole ribonucleotide synthetase from Escherichia coli.

    Abstract

    Aminoimidazole ribonucleotide (AIR) synthetase has been purified 15-fold to apparent homogeneity from Escherichia coli which contains a multicopy plasmid containing the purM, AIR synthetase, gene. The protein is a dimer composed of two identical subunits of Mr 38,500. The N-terminal sequence, amino acid composition, and steady-state kinetics of the protein have been determined. AIR synthetase has been shown to catalyze the transfer of the formyl oxygen of [18O]formylglycinamide ribonucleotide to Pi.

    PMID:
    3530323
    [PubMed - indexed for MEDLINE]

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