Although many source waterbodies face nitrogen pollution problems, the lack of organic electron donors causes difficulties when aerobic denitrifying bacteria are used to treat micro-polluted water. Different forms of iron with granular activated carbon (AC) as carriers were used to stimulate aboriginal microorganisms for the purification of micro-polluted source water. Compared with the iron-absent AC system, targeted pollutants were significantly removed (75.76% for nitrate nitrogen, 95.90% for total phosphorus, and 80.59% for chemical oxygen demand) in the sponge-iron-modified AC system, which indicated that iron promoted the physical and chemical removal of pollutants. In addition, high-throughput sequencing showed that bacterial distribution and interaction were changed by ion dosage, which was beneficial for pollutant transformation and reduction. Microbial functions, such as pollutant removal and expression of functional enzymes that were responsible for the transformation of nitrate nitrogen to ammonia, were highly efficient in iron-applied systems. This study provides an innovative strategy to strengthen in situ remediation of micro-pollution in waterbodies.
Keywords: aerobic denitrification; co-occurrence model; iron carbon; micro-pollution; microbial community.