Cell. 1986 Jun 20;45(6):827-35.

A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.

Abstract

Nuclear and cytoplasmic poly(A)-binding proteins have been purified from Saccharomyces cerevisiae, and antisera have been used to isolate a gene that encodes them. The gene occurs in a single copy on chromosome 5 and gives rise to a unique, unspliced 2.1 kb transcript. The nuclear protein appears to be derived from the cytoplasmic one by proteolytic cleavage into 53 and 17 kd polypeptides that remain associated during isolation. DNA sequence determination reveals four tandemly arrayed 90 amino acid regions of homology that probably represent poly(A)-binding domains. A 55 residue A-rich region upstream of the initiator methionine codon in the mRNA shows an affinity for poly(A)-binding protein comparable to that of poly(A)180-220, raising the possibility of feedback regulation of translation.

PMID:: 3518950; [PubMed - indexed for MEDLINE]

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Saccharomyces Genome Database

Supplemental Content

Related citations

Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export. [Genes Dev. 1999]
Uncoupling of the hnRNP Npl3p from mRNAs during the stress-induced block in mRNA export.
Krebber H, Taura T, Lee MS, Silver PA. Genes Dev. 1999 Aug 1; 13(15):1994-2004.
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence. [Mol Cell Biol. 1986]
mRNA polyadenylate-binding protein: gene isolation and sequencing and identification of a ribonucleoprotein consensus sequence.
Adam SA, Nakagawa T, Swanson MS, Woodruff TK, Dreyfuss G. Mol Cell Biol. 1986 Aug; 6(8):2932-43.
Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation. [Mol Cell Biol. 1998]
Pbp1p, a factor interacting with Saccharomyces cerevisiae poly(A)-binding protein, regulates polyadenylation.
Mangus DA, Amrani N, Jacobson A. Mol Cell Biol. 1998 Dec; 18(12):7383-96.
Review A history of poly A sequences: from formation to factors to function. [Prog Nucleic Acid Res Mol Biol. 2002]
Review A history of poly A sequences: from formation to factors to function.
Edmonds M. Prog Nucleic Acid Res Mol Biol. 2002; 71:285-389.
Review Tales of poly(A): a review. [Gene. 1990]
Review Tales of poly(A): a review.
Munroe D, Jacobson A. Gene. 1990 Jul 16; 91(2):151-8.

See reviews... See all...

Cited by over 100 PubMed Central articles

Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site. [PLoS One. 2011]
Pub1p C-terminal RRM domain interacts with Tif4631p through a conserved region neighbouring the Pab1p binding site.
Santiveri CM, Mirassou Y, Rico-Lastres P, Martínez-Lumbreras S, Pérez-Cañadillas JM. PLoS One. 2011; 6(9):e24481. Epub 2011 Sep 8.
Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion. [Mol Cell Biol. 2011]
Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion.
Kumar GR, Shum L, Glaunsinger BA. Mol Cell Biol. 2011 Aug; 31(15):3113-25. Epub 2011 Jun 6.
A yeast model for polyalanine-expansion aggregation and toxicity. [Mol Biol Cell. 2011]
A yeast model for polyalanine-expansion aggregation and toxicity.
Konopka CA, Locke MN, Gallagher PS, Pham N, Hart MP, Walker CJ, Gitler AD, Gardner RG. Mol Biol Cell. 2011 Jun 15; 22(12):1971-84. Epub 2011 Apr 20.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding ...
A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.
Cell. 1986 Jun 20 ;45(6):827-35.

PubMed
Inventing the randomized double-blind trial: the Nuremberg salt test of 1835.
Inventing the randomized double-blind trial: the Nuremberg salt test of 1835.
J R Soc Med. 2006 Dec ;99(12):642-3.

PubMed
Recent developments in StemBase: a tool to study gene expression in human and mu...
Recent developments in StemBase: a tool to study gene expression in human and murine stem cells.
BMC Res Notes. 2009 Mar 10 ;2:39.

PubMed
Study of stem cell function using microarray experiments.
Study of stem cell function using microarray experiments.
FEBS Lett. 2005 Mar 21 ;579(8):1795-801.

PubMed
DNA methylation protects hematopoietic stem cell multipotency from myeloerythroi...
DNA methylation protects hematopoietic stem cell multipotency from myeloerythroid restriction.
Nat Genet. 2009 Nov ;41(11):1207-15. Epub 2009 Oct 4 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to:

A single gene from yeast for both nuclear and cytoplasmic polyadenylate-binding proteins: domain structure and expression.

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID, Grant Support

Publication Types

MeSH Terms

Substances

Secondary Source ID

Grant Support

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

Supplemental Content

Related citations

Cited by over 100 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information