-
Nuclear antigens recognized by antibodies present in liver disease sera.
Nuclear and nuclear matrix proteins of HeLa cells were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis, and subsequently transferred onto nitrocellulose. Antibodies present in sera of patients with primary biliary cirrhosis and autoimmune chronic active hepatitis reacted with some of the blotted proteins. The antibodies were mainly directed against chromatin-associated proteins and protein constituents of discrete RNP particles. In addition, antibodies found in autoimmune liver disease sera detected a hitherto undescribed nuclear protein of 54 kD, and a nuclear matrix protein of approximately 150 kD. Antibodies recognizing a nuclear 25 kD doublet apparently constituted a marker antibody for autoimmune liver disease. Those directed at the 17 kD centromere protein were associated with the primary biliary cirrhosis-related CREST syndrome, while those recognizing La antigen were related to cases of sicca syndrome associated with autoimmune liver diseases.
PMID: 3516468 [PubMed - indexed for MEDLINE]
PMCID: PMC1577365
-
Cited by 5 PubMed Central articles
-
Significance of extractable nuclear antigens in childhood autoimmune liver disease.
Gregorio GV, Davies ET, Mieli-Vergani G, Vergani D.
Clin Exp Immunol. 1995 Nov; 102(2):308-13.
[Clin Exp Immunol. 1995]
-
High prevalence of antibodies to histones among patients with primary biliary cirrhosis.
Penner E, Muller S, Zimmermann D, Van Regenmortel MH.
Clin Exp Immunol. 1987 Oct; 70(1):47-52.
[Clin Exp Immunol. 1987]
-
HLA-DR antigens in systemic lupus erythematosus: association with specificity of autoantibody responses to nuclear antigens.
Smolen JS, Klippel JH, Penner E, Reichlin M, Steinberg AD, Chused TM, Scherak O, Graninger W, Hartter E, Zielinski CC.
Ann Rheum Dis. 1987 Jun; 46(6):457-62.
[Ann Rheum Dis. 1987]
- » See all...