Effect of alpha-1-antichymotrypsin on DNA synthesis in permeabilized human cells

M Tsuda; Y Umezawa; M Masuyama; K Yamaguchi; T Katsunuma

doi:10.1016/s0006-291x(87)80525-9

Effect of alpha-1-antichymotrypsin on DNA synthesis in permeabilized human cells

Biochem Biophys Res Commun. 1987 Apr 14;144(1):409-14. doi: 10.1016/s0006-291x(87)80525-9.

Authors

M Tsuda, Y Umezawa, M Masuyama, K Yamaguchi, T Katsunuma

PMID: 3495264
DOI: 10.1016/s0006-291x(87)80525-9

Abstract

The effect of Alpha-1-antichymotrypsin(ACT) on DNA synthesis was studied using lysolecithin-permeabilized cultured human stomach carcinoma cells. ACT added in medium inhibited DNA synthesis and the degree of inhibition is incubation time dependent. It is proportional to ACT concentration and the concentration of ACT required for 50% inhibition was 0.8 mg/ml.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / metabolism
Cell Line
Cell Membrane Permeability
DNA Polymerase II / antagonists & inhibitors
DNA, Neoplasm / biosynthesis*
Humans
Lysophosphatidylcholines
Stomach Neoplasms / metabolism
alpha 1-Antichymotrypsin / metabolism
alpha 1-Antichymotrypsin / pharmacology*

Substances

DNA, Neoplasm
Lysophosphatidylcholines
alpha 1-Antichymotrypsin
DNA Polymerase II