Human T lymphocytes express a protein-tyrosine kinase homologous to p56LSTRA

Biochim Biophys Acta. 1986 Oct 10;888(3):286-95. doi: 10.1016/0167-4889(86)90228-4.

Abstract

We have used oligonucleotide probes, based on a portion of the p60v-src autophosphorylation sequence, Glu-Asp-Asn-Glu-Tyr-Thr, to identify and characterize a cDNA from the human T-leukemia cell line, JURKAT. The JURKAT cDNA (designated ptk-JURKAT) was homologous to but distinct from the src, yes and fgr oncogenes, which encode protein-tyrosine kinases (ATP:protein phosphotransferase, EC 2.7.1.37). The ptk-JURKAT cDNA hybridized with a 2.2 kb RNA transcript from JURKAT cells and the human T-cell lymphoma line, MOLT-4, but failed to identify any transcript in two human B-cell lymphoma lines or a human erythroid-myeloid leukemia line, K562. Recently the nucleotide sequence has been established for the murine lymphocyte protein tyrosine kinase, p56LSTRA. The ptk-JURKAT cDNA appears to encode the human homolog of p56LSTRA.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • DNA / genetics
  • DNA, Neoplasm / genetics
  • Humans
  • Leukemia / enzymology
  • Lymphoma / enzymology*
  • Mice
  • Protein-Tyrosine Kinases / genetics*
  • Protein-Tyrosine Kinases / metabolism
  • Sequence Homology, Nucleic Acid
  • T-Lymphocytes / enzymology*

Substances

  • DNA, Neoplasm
  • DNA
  • Protein-Tyrosine Kinases

Associated data

  • GENBANK/X04476