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A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
The mRNAs of transiently expressed genes frequently contain an AU-rich sequence in the 3' untranslated region. We introduced a 51 nucleotide AT sequence from a human lymphokine gene, GM-CSF, into the 3' untranslated region of the rabbit beta-globin gene. Our experiments demonstrate that this caused the otherwise stable beta-globin mRNA to become highly unstable in vivo. The instability conferred by the AU sequence in the mRNA was partially alleviated by treatment of the cells with cycloheximide. We propose that the AU sequences are the recognition signal for an mRNA processing pathway which specifically degrades the mRNAs for certain lymphokines, cytokines, and proto-oncogenes.
PMID: 3488815 [PubMed - indexed for MEDLINE]
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Cited by over 100 PubMed Central articles
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Database for mRNA half-life of 19 977 genes obtained by DNA microarray analysis of pluripotent and differentiating mouse embryonic stem cells.
Sharova LV, Sharov AA, Nedorezov T, Piao Y, Shaik N, Ko MS.
DNA Res. 2009 Feb; 16(1):45-58. Epub 2008 Nov 11.
[DNA Res. 2009]
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Oncogenic Ras and transforming growth factor-beta synergistically regulate AU-rich element-containing mRNAs during epithelial to mesenchymal transition.
Kanies CL, Smith JJ, Kis C, Schmidt C, Levy S, Khabar KS, Morrow J, Deane N, Dixon DA, Beauchamp RD.
Mol Cancer Res. 2008 Jul; 6(7):1124-36.
[Mol Cancer Res. 2008]
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Patterns of evolution and host gene mimicry in influenza and other RNA viruses.
Greenbaum BD, Levine AJ, Bhanot G, Rabadan R.
PLoS Pathog. 2008 Jun 6; 4(6):e1000079. Epub 2008 Jun 6.
[PLoS Pathog. 2008]
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