miR-199a Targeting PNRC1 to Promote Keratinocyte Proliferation and Invasion in Cholesteatoma

Biomed Res Int. 2021 Nov 16:2021:1442093. doi: 10.1155/2021/1442093. eCollection 2021.

Abstract

Introduction: miR-199a has been reported as an oncogene of various cancers. However, the biological function and regulatory mechanism of miR-199a in keratinocytes of cholesteatoma are still unclear.

Methods: Detection by qRT-PCR was conducted on miR-199a's expression in both thirty pairs of cholesteatoma tissues and normal skins. For characterizing the function of miR-199a, this research adopted transwell assay, wound healing assay, and CCK8 assays. Under the support of qRT-PCR, efforts were made to investigate the relative expression of candidate target genes. Moreover, the evaluation of the targeting relationship between miR-199a and the candidate target gene was conducted with the dual-luciferase reporter assay.

Results: The upregulation of miR-199a was found in cholesteatoma tissues, which facilitated the proliferation, migration, and invasion of HaCaT cells, while its downregulation caused opposite results.

Conclusions: The findings of the present research offer more insights into the molecular mechanism of cholesteatoma progression.

MeSH terms

  • Cell Line
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • Cholesteatoma, Middle Ear / genetics*
  • Cholesteatoma, Middle Ear / pathology*
  • Gene Expression
  • Humans
  • Keratinocytes / pathology*
  • MicroRNAs / genetics*
  • Nuclear Proteins / genetics*
  • Transcription Factors / genetics*
  • Up-Regulation

Substances

  • MicroRNAs
  • Nuclear Proteins
  • PNRC1 protein, human
  • Transcription Factors
  • mirn199 microRNA, human