Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer

Kui Xiao; Yang Wang; Lihua Zhou; Jufen Wang; Yaohui Wang; De Tong; Zhiruo Zhu; Jiehan Jiang

doi:10.1371/journal.pone.0259091

Construction of ceRNA network to identify the lncRNA and mRNA related to non-small cell lung cancer

PLoS One. 2021 Oct 29;16(10):e0259091. doi: 10.1371/journal.pone.0259091. eCollection 2021.

Authors

Kui Xiao^{1

2

3}, Yang Wang⁴, Lihua Zhou⁵, Jufen Wang⁵, Yaohui Wang^{1

2

3}, De Tong^{1

2

3}, Zhiruo Zhu^{1

2

3}, Jiehan Jiang⁵

Affiliations

¹ Department of Respiratory and Critical Care Medicine, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
² Research Unit of Respiratory Disease, Central South University, Changsha, Hunan, China.
³ The Respiratory Disease Diagnosis and Treatment Center of Hunan Province, Changsha, Hunan, China.
⁴ Department of Pathology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
⁵ Department of Pulmonary and Critical Care Medicine, University of South China Affiliated Changsha Central Hospital, Changsha City, Hunan Province, China.

Abstract

Background: Non-small cell lung cancer (NSCLC) harms human health, but its pathogenesis remains unclear. We wish to provide more molecular therapeutic targets for NSCLC.

Methods: The NSCLC tissue and normal tissue samples were screened for genetic comparison in the TCGA database. The predicted lncRNA and mRNA in BEAS2B and A549 cells were detected.

Results: Volcano plot displayed differentially expressed lncRNAs and mRNAs in adjacent tissues and NSCLC tissues. The survival curve showed that the lncRNA and mRNA had a significant impact on the patient's survival. The results of GO term enrichment analysis indicated that mRNA functions were enriched in cell cycle-related pathways. In the ceRNA interaction network, 13 lncRNAs and 20 miRNAs were found to have an interactive relationship. Finally, 3 significantly different lncRNAs (LINC00968, lnc-FAM92A-9 and lnc-PTGFR-1) and 6 mRNAs (CTCFL, KRT5, LY6D, TMEM, GBP6, and TMEM179) with potential therapeutic significance were screened out. And the cell experiment verified our results.

Conclusion: We screened out clinically significant 3 lncRNAs and 6 mRNAs involved in the ceRNA network, which were the key to our future research on the treatment of NSCLC.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

A549 Cells
Biomarkers, Tumor / genetics*
Carcinoma, Non-Small-Cell Lung / genetics*
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
Humans
Lung Neoplasms / genetics*
RNA, Long Noncoding / physiology*
RNA, Messenger / physiology*

Substances

Biomarkers, Tumor
RNA, Long Noncoding
RNA, Messenger

Grants and funding

This work was supported by the Scientific Research Project of Hunan Provincial Health Commission [No. 202103020704]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.