Objective: To establish a mouse model of respiratory syncytial virus (RSV) infection after hematopoietic stem cell transplantation, so as to lay the foundation for future research on RSV infection and related complications after hematopoietic stem cell transplantation.
Methods: Bone marrow cells and spleen cells were transplanted to C57BL/6 mice after myeloablative treatment to establish a mouse model of allogeneic hematopoietic stem cell transplantation. The chimerism rate was detected by flow cytometry 3 and 7 weeks after transplantation. The transplanted mice were infected with RSV by nasal drops. The lung tissues were collected 5 days after infection for identification of infection, and lung tissues were analyzed for pathology 2 weeks and 2 months after infection.
Results: The chimerism rate was > 90% at 3 and 7 weeks after transplantation. Successful infection was detected 5 days after RSV infection, and there were severe and persistent pathological changes in the lung tissues of the mice 2 weeks and 2 months after infection.
Conclusion: RSV infection in stable chimeric mice after hematopoietic stem cell transplantation can cause significantly persistent lung disease, which lays foundation for the prevention and treatment of RSV infection and the mechanism of later bronchiolitis obliterans after hematopoietic stem cell transplantation.
题目: 呼吸道合胞病毒感染造血干细胞移植小鼠模型的建立.
目的: 建立呼吸道合胞病毒（RSV）感染造血干细胞移植后小鼠模型，为今后造血干细胞移植后RSV感染及相关并发症的研究奠定基础。.
方法: 采用骨髓细胞和脾细胞移植给经清髓性处理后的C57BL/6小鼠，建立同种异体造血干细胞移植小鼠模型，移植后3周及7周通过流式细胞术检测嵌合率。通过滴鼻对移植后小鼠进行RSV感染，取感染后5 d肺组织进行感染鉴定、感染后2周及2个月肺组织进行病理分析。.
结果: 移植后3周及7周嵌合率均>90%。在RSV感染后5 d可检测到感染成功，且感染后2周及2个月小鼠肺组织均存在严重、持续的病理改变。.
结论: RSV感染稳定嵌合的造血干细胞移植小鼠，可导致显著持久肺部病变，为造血干细胞移植后RSV感染防治及后期闭塞性细支气管炎发生机制的研究奠定基础。.