Preparation of desulphated bovine fibrinopeptide B and demonstration of its sulphation in vitro by an enzyme system from neuroblastoma-glioma hybrid cells

Int J Biochem. 1986;18(2):137-41. doi: 10.1016/0020-711x(86)90145-x.

Abstract

A method is described for isolating bovine fibrinopeptide B (bFPB) in a highly purified form from crude bovine fibrinogen, using ion-exchange chromatography on DEAE cellulose. Desulphated bFPB (designated DSbFPB) was prepared by treatment of the product with acid. After incubating DSbFPB with [35S]PAPS, in the presence of a particulate preparation from neuroblastoma-glioma hybrid cells, radioactivity was incorporated into a product identified as [35S]bFPB from its position of elution on reverse-phase HPLC. The possible significance of this observation is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acids / analysis
  • Animals
  • Cattle
  • Cell Line
  • Chromatography, High Pressure Liquid
  • Fibrinogen / isolation & purification*
  • Fibrinopeptide A / isolation & purification
  • Fibrinopeptide B / isolation & purification*
  • Fibrinopeptide B / metabolism
  • Glioma / enzymology*
  • Hybrid Cells / enzymology*
  • Mice
  • Neuroblastoma / enzymology*
  • Rats
  • Sulfates
  • Sulfurtransferases / metabolism

Substances

  • Amino Acids
  • Sulfates
  • Fibrinopeptide A
  • Fibrinopeptide B
  • Fibrinogen
  • Sulfurtransferases