Explanations accounting for transduction by bacteriophage lambda in maltose negative bacteriophage lambda resistant mutants of Escherichia coli K-12

Mol Gen Genet. 1978 Feb 16;159(2):143-9. doi: 10.1007/BF00270887.

Abstract

Entry of DNA from lambda phages particles into lambdarMAl- mutants of Escherichia coli K-12 is shown to be due to two distinguishable processes. One, residual transduction, results from a low level expression of lamB. The other one, background transduction, is independent of gene lamB. Interpretations are presented for these results. It is propos that residual transduction is due to a weak promoter pB3 located within or near the distal part of the gene preceeding lamB in the same operon. It is proposed that background transduction is due to a secondary receptor structure for phage lambda. Finally a tentative hypothesis relatin pB3 to insertion sequences is presented.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Coliphages / genetics*
  • Coliphages / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Genes*
  • Maltose / genetics
  • Maltose / metabolism*
  • Mutation
  • Transduction, Genetic*

Substances

  • Maltose