lncRNA PANTR1 Upregulates BCL2A1 Expression to Promote Tumorigenesis and Warburg Effect of Hepatocellular Carcinoma through Restraining miR-587

J Immunol Res. 2021 Aug 12:2021:1736819. doi: 10.1155/2021/1736819. eCollection 2021.

Abstract

Hepatocellular carcinoma (HCC) is one of the most common subtypes of malignant liver tumors, characterized by high morbidity and mortality. Due to its poor diagnosis strategy and inefficient clinical intervention, HCC has brought terrible life experiences for patients worldwide. Finding novel curative agents for HCC is urgently needed. In the current study, we hypothesized that lncRNA PANTR1 participates in HCC initiation or progression. Our study found that lncRNA PANTR1 was upregulated in HCC tumor tissues and abundantly expressed in HCC cell lines. PANTR1 knockdown inhibited cell growth and migration, promoted cell apoptosis in vitro, and suppressed tumor cell growth in vivo. Moreover, our results suggest that downregulated PANTR1 inhibited the Warburg effect in HCC cells. Underlying mechanisms of PANTR1 in HCC progression were investigated. PANTR1 acted as a competent sponge for miR-587 and downregulated miR-587 expression in HCC cells. Further, MiR-587 directly targets BCL2A1. lncRNA PANTR1 promotes HCC progression via mediating the miR-587-BCL2A1 axis. Our study identified a novel lncRNA PANTR1/miR-587/BCL2A1 axis in HCC progression. We might provide a new target for HCC basic research and clinical management.

MeSH terms

  • 3' Untranslated Regions
  • Animals
  • Apoptosis / genetics
  • Carcinoma, Hepatocellular / genetics*
  • Carcinoma, Hepatocellular / metabolism*
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Transformation, Neoplastic
  • Disease Models, Animal
  • Fluorescent Antibody Technique
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Liver Neoplasms / genetics*
  • Liver Neoplasms / metabolism*
  • Mice
  • MicroRNAs / genetics*
  • Minor Histocompatibility Antigens / genetics*
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • RNA Interference
  • RNA, Long Noncoding / genetics*
  • Warburg Effect, Oncologic

Substances

  • 3' Untranslated Regions
  • BCL2-related protein A1
  • MIRN587 microRNA, human
  • MicroRNAs
  • Minor Histocompatibility Antigens
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Long Noncoding