Detoxification of aflatoxin B₁ in broiler chickens by a triple-action feed additive

The aim of this study was to evaluate the detoxification of aflatoxin B₁ (AFB₁) in vitro and in broiler chickens using a triple-action compound mycotoxin detoxifier (CMD). Response surface methodology (RSM) was used to evaluate AFB1 detoxification in artificial gastrointestinal fluid (AGIF) in vitro. The AFB₁-degradation rate was 41.5% (P < .05) when using a compound probiotic (CP) in which the visible counts of Bacillus subtilis, Lactobacillus casein, Enterococcus faecalis and Candida utilis were 1.0 × 10⁵, 1.0 × 10⁵, 1.0 × 10⁷ and 1.0 × 10⁵ CFU/mL, respectively. When CP was combined with 0.1% AFB₁-degrading enzyme to give CPADE, the AFB₁-degradation rate was increased to 55.28% (P < .05). The AFB₁-removal rate was further increased to above 90% when CPADE was combined with 0.03% montmorillonite to make CMD. In vivo, a total of 150 one-day-old Ross broilers were allotted to 3 groups, 5 replications for each group, 10 broilers in each replication. Group A: basal diet, Group B: basal diet with 40 μg/kg AFB₁, Group C: basal diet with 40 μg/kg AFB₁ plus CMD. The feeding experiment period was 21 d. The results showed that broiler growth was increased, and AFB₁ residues in serum, excreta and liver were decreased by CMD addition in broiler diet (P < .05). In conclusion, CMD was able to remove AFB₁ efficiently in vitro and to increase broiler production performance and reduce AFB₁ residues in the chickens.