Comparative proteomics elucidates the dynamics of ovarian development in the Chinese mitten crab Eriocheir sinensis

Qiang-Mei Feng; Mei-Mei Liu; Yong-Xu Cheng; Xu-Gan Wu

doi:10.1016/j.cbd.2021.100878

Comparative proteomics elucidates the dynamics of ovarian development in the Chinese mitten crab Eriocheir sinensis

Comp Biochem Physiol Part D Genomics Proteomics. 2021 Dec:40:100878. doi: 10.1016/j.cbd.2021.100878. Epub 2021 Jul 8.

Authors

Qiang-Mei Feng¹, Mei-Mei Liu², Yong-Xu Cheng³, Xu-Gan Wu⁴

Affiliations

¹ Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China.
² Key Laboratory of Marine Biotechnology of Jiangsu Province, Jiangsu Ocean University, Lianyungang 222005, China. Electronic address: mmliu@jou.edu.cn.
³ Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China.
⁴ Centre for Research on Environmental Ecology and Fish Nutrition of Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306, China; National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China. Electronic address: xgwu@shou.edu.cn.

PMID: 34333232
DOI: 10.1016/j.cbd.2021.100878

Abstract

Ovarian development is a complex physiological process for crustacean reproduction that is divided into the oogonium proliferation stage, endogenous vitellogenic stage, exogenous vitellogenic stage, and oocyte maturation stage. Proteomics analysis offers a feasible approach to reveal the proteins involved in the complex physiological processes of any organism. Therefore, this study performed a comparative proteomics analysis of the ovary and hepatopancreas at three key ovarian stages, including stages I (oogonium proliferation), II (endogenous vitellogenesis) and IV (exogenous vitellogenesis), of the Chinese mitten crab Eriocheir sinensis using a label-free quantitative approach. The results showed that a total of 2,224 proteins were identified, and some key proteins related to ovarian development and nutrition metabolism were differentially expressed. The 26 key proteins were mainly involved in the ubiquitin/proteasome pathway (UPP), cyclic AMP-protein kinase A (cAMP-PKA) signaling pathway, and mitogen-activated protein kinase (MAPK) signaling pathway during oogenesis. Fifteen differentially abundant proteins (DAPs) were found to participate in vitellogenesis and oocyte development, such as vitelline membrane outer layer protein 1 homolog, vitellogenin, vitellogenin receptor, heat shock 70 kDa protein cognate 3 and farnesyl pyrophosphate synthase. Forty-seven DAPs related to nutrition metabolism were identified, including the protein digestion, fatty acid metabolism, prostaglandin metabolism, lipid digestion and transportation, i.e. short-chain specific acyl-CoA dehydrogenase, acyl-CoA desaturase, fatty acid-binding protein, long-chain fatty acid CoA ligase 4, and hematopoietic prostaglandin D synthase. These results not only indicate proteins involved in ovarian development and nutrient deposition but also enhance the understanding of the regulatory pathways and physiological processes of crustacean ovarian development.

Keywords: Comparative proteomics; Crustacean; Nutrient deposition; Ovarian development; Vitellogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brachyura*
China
Female
Hepatopancreas
Ovary
Proteomics*
Vitellogenesis