Proc Natl Acad Sci U S A. 1988 Mar;85(5):1492-6.

Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

Bonthron DT, Morton CC, Orkin SH, Collins T.

Source

Division of Hematology-Oncology, Children's Hospital, Boston, MA.

Abstract

Genomic clones encoding the A chain of platelet-derived growth factor (PDGF) have been isolated. The gene contains seven exons spanning about 24 kilobases of DNA. The positions of intervening sequences closely match those of the related B-chain (c-sis) gene on chromosome 22. In situ hybridization was used to localize the PDGF A-chain gene to the distal portion of the short arm of chromosome 7 (7p21-p22). Within the (G + C)-rich 5' region, a single transcriptional start site was identified approximately equal to 36 base pairs downstream of a TATAA consensus promoter element. The three size classes of A-chain mRNA probably arise by selection of alternative poly(A) sites in exon 7, but only a single consensus AATAAA signal was identified in this region. Two functionally different A-chain precursors, which differ by the presence or absence of a basic C terminus, are generated as a result of alternative mRNA splicing events, which include or exclude exon 6. This and other structural features of the A-chain gene suggest that PDGF expression may be modulated at transcriptional and post-transcriptional levels.

PMID:: 3422746; [PubMed - indexed for MEDLINE]
PMCID:: PMC279797

Free PMC Article

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene. [Proc Natl Acad Sci U S A. 1991]
Gene structure, chromosomal location, and basis for alternative mRNA splicing of the human VCAM1 gene.
Cybulsky MI, Fries JW, Williams AJ, Sultan P, Eddy R, Byers M, Shows T, Gimbrone MA Jr, Collins T. Proc Natl Acad Sci U S A. 1991 Sep 1; 88(17):7859-63.
Structure, 5'-flanking sequence, and chromosome location of the human N-formyl peptide receptor gene. A single-copy gene comprised of two exons on chromosome 19q.13.3 that yields two distinct transcripts by alternative polyadenylation. [Biochemistry. 1993]
Structure, 5'-flanking sequence, and chromosome location of the human N-formyl peptide receptor gene. A single-copy gene comprised of two exons on chromosome 19q.13.3 that yields two distinct transcripts by alternative polyadenylation.
Haviland DL, Borel AC, Fleischer DT, Haviland JC, Wetsel RA. Biochemistry. 1993 Apr 27; 32(16):4168-74.
Splicing of the platelet-derived-growth-factor A-chain mRNA in human malignant mesothelioma cell lines and regulation of its expression. [Eur J Biochem. 1992]
Splicing of the platelet-derived-growth-factor A-chain mRNA in human malignant mesothelioma cell lines and regulation of its expression.
Langerak AW, Dirks RP, Versnel MA. Eur J Biochem. 1992 Sep 15; 208(3):589-96.
Promoter region of the human platelet-derived growth factor A-chain gene. [Proc Natl Acad Sci U S A. 1991]
Promoter region of the human platelet-derived growth factor A-chain gene.
Takimoto Y, Wang ZY, Kobler K, Deuel TF. Proc Natl Acad Sci U S A. 1991 Mar 1; 88(5):1686-90.
Review Transcriptional control of the platelet-derived growth factor subunit genes. [Biofactors. 1993]
Review Transcriptional control of the platelet-derived growth factor subunit genes.
Kaetzel DM, Coyne DW, Fenstermaker RA. Biofactors. 1993 May; 4(2):71-81.

See reviews... See all...

Cited by 26 PubMed Central articles

PDGF-A promoter and enhancer elements provide efficient and selective antineoplastic gene therapy in multiple cancer types. [Cancer Gene Ther. 2009]
PDGF-A promoter and enhancer elements provide efficient and selective antineoplastic gene therapy in multiple cancer types.
Mishra A, Ormerod AK, Cibull ML, Spear BT, Kraner SD, Kaetzel DM. Cancer Gene Ther. 2009 Apr; 16(4):298-309. Epub 2008 Nov 7.
NF1/X represses PDGF A-chain transcription by interacting with Sp1 and antagonizing Sp1 occupancy of the promoter. [EMBO J. 2002]
NF1/X represses PDGF A-chain transcription by interacting with Sp1 and antagonizing Sp1 occupancy of the promoter.
Rafty LA, Santiago FS, Khachigian LM. EMBO J. 2002 Feb 1; 21(3):334-43.
Early growth response factor-1 induction by injury is triggered by release and paracrine activation by fibroblast growth factor-2. [Am J Pathol. 1999]
Early growth response factor-1 induction by injury is triggered by release and paracrine activation by fibroblast growth factor-2.
Santiago FS, Lowe HC, Day FL, Chesterman CN, Khachigian LM. Am J Pathol. 1999 Mar; 154(3):937-44.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (nucleotide/PMC)
Records in Gene identified from shared sequence and PMC links.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Platelet-derived growth factor A chain: gene structure, chromosomal location, an...
Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.
Proc Natl Acad Sci U S A. 1988 Mar ;85(5):1492-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to:

Platelet-derived growth factor A chain: gene structure, chromosomal location, and basis for alternative mRNA splicing.

Source

Abstract

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

MeSH Terms

Substances

Secondary Source ID

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Cited by 26 PubMed Central articles

Related information

Recent activity

Simple NCBI Directory

Getting Started

Resources

Popular

Featured

NCBI Information