Altered expression of SARS-CoV-2 entry and processing genes by Porphyromonas gingivalis-derived lipopolysaccharide, inflammatory cytokines and prostaglandin E2 in human gingival fibroblasts

Kotaro Sena; Kirara Furue; Fumiaki Setoguchi; Kazuyuki Noguchi

doi:10.1016/j.archoralbio.2021.105201

Altered expression of SARS-CoV-2 entry and processing genes by Porphyromonas gingivalis-derived lipopolysaccharide, inflammatory cytokines and prostaglandin E₂ in human gingival fibroblasts

Arch Oral Biol. 2021 Sep:129:105201. doi: 10.1016/j.archoralbio.2021.105201. Epub 2021 Jun 21.

Authors

Kotaro Sena¹, Kirara Furue², Fumiaki Setoguchi³, Kazuyuki Noguchi⁴

Affiliations

¹ Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: senaperi@dent.kagoshima-u.ac.jp.
² Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: kiraraf@dent.kagoshima-u.ac.jp.
³ Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: f-seto@d1.dent.kagoshima-u.ac.jp.
⁴ Department of Periodontology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima, Japan. Electronic address: kazuperi@dent.kagoshima-u.ac.jp.

Abstract

Objective: The aim of this in vitro study was to investigate the expression of SARS-CoV-2 entry and processing genes in human gingival fibroblasts (HGnF) following treatment with Porphyromonas gingivalis-derived lipopolysaccharide (PgLPS) or inflammatory cytokines/mediators.

Design: We assessed the expression of SARS-CoV-2 entry and processing genes; angiotensin-converting enzyme 2 (ACE2), cellular serine proteases transmembrane serine protease 2 (TMPRSS2), Furin, and basigin (BSG) in HGnF by real-time PCR. To further asses the contribution of PgLPS and inflammatory cytokines/mediators to proliferation and SARS-CoV-2 entry and processing gene expression, HGnF were treated with PgLPS, IL1β, TNFα, and PGE₂.

Results: The expression for ACE2 in HGnF was significantly elevated after PgLPS or IL1β, TNFα, PGE₂ treatment. The expression of TMPRSS2 was increased by PgLPS, IL1β, or PGE₂ while BSG was elevated by PgLPS and IL1β. The expression of BSG and FURIN decreased after TNFα treatment.

Conclusion: SARS-CoV-2 entry and processing genes are expressed in human gingival fibroblasts and their expressions are altered by PgLPS, IL1β, TNFα and PGE₂ treatment.

Keywords: Angiotensin-converting enzyme 2; Basigin; COVID-19; Furin; Transmembrane serine protease 2.

MeSH terms

COVID-19*
Cytokines
Fibroblasts
Humans
Lipopolysaccharides / pharmacology
Porphyromonas gingivalis
Prostaglandins
Prostaglandins E
SARS-CoV-2*

Substances

Cytokines
Lipopolysaccharides
Prostaglandins
Prostaglandins E