Neobacillus massiliamazoniensis sp. nov., a new bacterial species isolated from stool sample of an inhabitant of the Amazon region

B Mbaye; M Tidjani Alou; A Fadlane; L Fregiere; S Alibar; M Million; F Fenollar; C I Lo

doi:10.1016/j.nmni.2021.100900

Neobacillus massiliamazoniensis sp. nov., a new bacterial species isolated from stool sample of an inhabitant of the Amazon region

New Microbes New Infect. 2021 May 18:42:100900. doi: 10.1016/j.nmni.2021.100900. eCollection 2021 Jul.

Authors

B Mbaye^{1

2}, M Tidjani Alou^{1

2}, A Fadlane^{1

2}, L Fregiere^{1

2}, S Alibar^{1

2}, M Million^{1

2}, F Fenollar^{2

3}, C I Lo^{2

3}

Affiliations

¹ Aix Marseille Univ, IRD, AP-HM, MEPHI, Marseille, France.
² IHU-Méditerranée Infection, Marseille, France.
³ Aix Marseille Univ, IRD, AP-HM, SSA, VITROME, Marseille, France.

Abstract

Using a culturomics approach, a strain was isolated, identified and characterised following the taxonogenomics concept. Neobacillus massiliamazoniensis sp. nov., strain LF1^T (=CSURP1359) was isolated from human stool. The 16S rRNA gene sequence analysis of strain LF1^T (accession number: LK021124) exhibits 98.32% similarity levels with Neobacillus bataviensis strain IDA1115 (accession number: NR_036766.1), the phylogenetically closest related species with standing in nomenclature. The draft genome size of strain LF1^T (accession number: CVRB00000000) is 4.6 Mbp with a G+C content of 34.1 mol%. Analysis of phylogenic tree, genomic analysis and phenotypic criteria described here sufficiently prove that this bacterium is different from previously known bacterial species with standing in nomenclature and represents a new Neobacillus species belonging to Firmicutes phylum.

Keywords: Culturomics; Neobacillus massiliamazoniensis; genome; gut microbiota; taxonogenomics.