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FEBS Lett. 1988 Aug 29;236(2):352-6.

Cloning and complete amino acid sequences of human and murine basement membrane protein BM-40 (SPARC, osteonectin).

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  • 1Dermatologische Klinik der Universit√§t M√ľnchen, FRG.


Amino acid sequences of 285 and 286 residues, respectively, were deduced for mouse and human BM-40 from cDNA clones isolated from expression libraries. The sequences showed 92% identity and were also essentially identical to those of bone osteonectin and of the parietal endoderm protein SPARC. About 60% of the mouse BM-40 sequence was confirmed by Edman degradation. Two of the seven disulfide bonds were localized which apparently separate two distinct domains of mouse BM-40.

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