L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma

Maia Norman; Dmitry Ter-Ovanesyan; Wendy Trieu; Roey Lazarovits; Emma J K Kowal; Ju Hyun Lee; Alice S Chen-Plotkin; Aviv Regev; George M Church; David R Walt

doi:10.1038/s41592-021-01174-8

L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma

Nat Methods. 2021 Jun;18(6):631-634. doi: 10.1038/s41592-021-01174-8. Epub 2021 Jun 3.

Authors

Maia Norman^#^{1

2

3}, Dmitry Ter-Ovanesyan^#², Wendy Trieu², Roey Lazarovits², Emma J K Kowal⁴, Ju Hyun Lee², Alice S Chen-Plotkin⁵, Aviv Regev^{4

6}, George M Church^{2

7}, David R Walt^{8

9

10}

Affiliations

¹ Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA.
² Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA.
³ Tufts University School of Medicine, Boston, MA, USA.
⁴ Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
⁵ Department of Neurology, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
⁶ Broad Institute of MIT and Harvard, Cambridge, MA, USA.
⁷ Harvard Medical School, Boston, MA, USA.
⁸ Department of Pathology, Brigham and Women's Hospital, Boston, MA, USA. dwalt@bwh.harvard.edu.
⁹ Wyss Institute for Biologically Inspired Engineering at Harvard University, Boston, MA, USA. dwalt@bwh.harvard.edu.
¹⁰ Harvard Medical School, Boston, MA, USA. dwalt@bwh.harvard.edu.

^# Contributed equally.

Abstract

L1CAM is a transmembrane protein expressed on neurons that was presumed to be found on neuron-derived extracellular vesicles (NDEVs) in human biofluids. We developed a panel of single-molecule array assays to evaluate the use of L1CAM for NDEV isolation. We demonstrate that L1CAM is not associated with extracellular vesicles in human plasma or cerebrospinal fluid and therefore recommend against its use as a marker in NDEV isolation protocols.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / metabolism
Centrifugation
Chromatography, Gel
Culture Media, Conditioned
Extracellular Vesicles / metabolism*
Humans
Neural Cell Adhesion Molecule L1 / blood
Neural Cell Adhesion Molecule L1 / cerebrospinal fluid
Neural Cell Adhesion Molecule L1 / metabolism*
Neurons / metabolism

Substances

Biomarkers
Culture Media, Conditioned
L1CAM protein, human
Neural Cell Adhesion Molecule L1

Abstract

Publication types

MeSH terms

Substances

Grants and funding