Planktonic microbial responses to perfluorinated compound (PFC) pollution: Integrating PFC distributions with community coalescence and metabolism

The presence of perfluorinated compound (PFC) contamination in riverine ecosystems represents a novel challenge for environmental remediation. However, little attention has been paid to how PFCs affect planktonic microbial community coalescence. Here, the spatial profiles of fourteen PFCs and their contributions to community assembly were determined using field sampling in a natural river confluence. Overall, PFPeA (perfluorovaleric acid), PFBS (perfluorobutylsulfonate), PFHpA (perfluoroheptanoic acid) and PFHxA (perfluorohexanoic acid) were identified as important indicators of PFC pollution, accounting for the majority of the spatial heterogeneity in PFC pollution. PFPeA (perfluorovaleric acid) (9.39%) and PFTrDA (perfluorotridecanoate acid) (8.61%) contributed more to microbial taxonomic spatial heterogeneity than did other factors, such as pH, dissolved oxygen and velocity. PFOA (pentadecafluorooctanoic acid) (R² = 0.353) and PFBS (R² = 0.297) drove turnover in archaeal communities within river sections (transversely), while PFHpA (R² = 0.251) and PFOS (perfluorooctane sulphonate) (R² = 0.105) drove turnover in bacterial communities transversely and longitudinally, respectively. Phylogenetic null modeling suggested that archaeal (68.89-83.33%) community assembly was dominated by stochastic processes, and was balanced by PFHxA (R² = 0.349) and PFOA (R² = 0.290). Furthermore, PFOS inhibited the biosynthesis of several key amino acids in archaea, and PFBA enhanced the potential for bacterial infections in humans (p < 0.05), threatening water quality. In sum, this study provides new insights into riverine ecological risk management.