Send to

Choose Destination
See comment in PubMed Commons below
Biophys J. 1988 Jun;53(6):885-92.

Capacitance measurements. An analysis of the phase detector technique used to study exocytosis and endocytosis.

Author information

  • 1Department of Physiology, University of Pennsylvania, Philadelphia 19104-6085.


We have studied the admittance of patch-clamped mast cells during exocytosis and found that they are adequately described by a four parameter equivalent circuit. On the basis of these measurements, we show that, contrary to current belief, when using a phase sensitive detector, small capacitance changes due to exocytosis or endocytosis should be studied by measuring current 90 degrees out of phase, relative to the component that corresponds to changes in series resistance. We have extended the theory on phase-detectors to include the errors in the estimation of step changes of membrane capacitance. We show that the measured capacitance of a secretory granule can be up to 80% smaller than its true value, during the course of a typical mast cell degranulation. We also describe a software-based phase-detector that simplifies capacitance measurements.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk