Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

Methods Mol Biol. 2021:2305:193-201. doi: 10.1007/978-1-0716-1406-8_10.

Abstract

In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.

Keywords: Microtubule-associated proteins; Microtubules; Protein dynamics; Protein interactions; Solid-state NMR spectroscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy / methods*
  • Microtubule-Associated Proteins / chemistry
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / chemistry
  • Microtubules / metabolism*
  • Protein Conformation
  • Protein Interaction Domains and Motifs
  • Tubulin / chemistry
  • Tubulin / metabolism*

Substances

  • Microtubule-Associated Proteins
  • Tubulin