New triterpenoid saponins from the leaves of Ilex chinensis and their hepatoprotective activity

Chin J Nat Med. 2021 May;19(5):376-384. doi: 10.1016/S1875-5364(21)60036-5.

Abstract

Seven new triterpenoid saponins, including five ursane-type saponins, ilexchinenosides R-V (1-5), and two oleanane-type saponins, ilexchinenosides W-X (6-7), with four known triterpenoid saponins (8-11) were isolated from the leaves of Ilex chinensis. Their structures were elucidated by comprehensive spectroscopic 1D and 2D NMR and HR-ESI-MS data. Their sugar moieties were determined by HPLC analysis compared with standards after hydrolysis and derivatization. Compounds 1, 2, 4, 9 and 10 exhibited potential hepatoprotective activity against N-acetyl-p-aminophenol (APAP)-induced HepG2 cell injury in vitro.

Keywords: Hepatoprotective activity; Ilex chinensis; Triterpenoid saponins; Ursane and oleanane.

MeSH terms

  • Hep G2 Cells
  • Humans
  • Ilex* / chemistry
  • Liver / drug effects
  • Molecular Structure
  • Phytochemicals / pharmacology
  • Plant Leaves / chemistry
  • Protective Agents / pharmacology*
  • Saponins* / pharmacology
  • Triterpenes* / pharmacology

Substances

  • Phytochemicals
  • Protective Agents
  • Saponins
  • Triterpenes