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    FEBS Lett. 1988 Jul 18;234(2):395-9.

    Isolation of cDNA clones of human argininosuccinate lyase and corrected amino acid sequence.

    Source

    Department of Biochemistry, Faculty of Medicine, Kagoshima Univeristy, Japan.

    Abstract

    In the present study, we isolated clones of human argininosuccinate lyase (ASL) cDNA from a liver cDNA library using a clone of rat ASL cDNA and analyzed human ASL cDNA nucleotide sequence. The results reveal that the sequence of human ASL cDNA published by O'Brien et al. in 1986 [Proc. Natl. Acad. Sci USA 83, 7211-7215] had one-base deletions at three independent positions in the coding regions near the COOH-terminus, which caused frame-shift variations in the amino acid sequence. Amino acid sequencing of peptides prepared from purified human liver ASL showed our predicted amino acid sequence to be correct.

    PMID:
    3391281
    [PubMed - indexed for MEDLINE]

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