Oxidative stress, DNA damage, and cellular response in hydrogen peroxide-induced cell injury of mud crab (Scylla paramamosain)

Oxidative stress is considered as the toxicity mechanism of environmental stressors on aquatic organisms. This study aims to explore the effects of oxidative stress on physiological responses, DNA damage and transcriptional profiles of the mud crabs Scylla paramamosain. In the present study, mud crabs were injected with 0.1% and 1% hydrogen peroxide (H₂O₂) for 72 h. The results showed that superoxide dismutase and catalase activities significantly decreased after H₂O₂ injection. Malondialdehyde content, H₂O₂ content, aspartate aminotransferase, alanine aminotransferase and lactate dehydrogenase activity significantly increased after H₂O₂ injection. Moreover, DNA damage occurred after H₂O₂ injection. Transcriptome analysis showed that 531 and 372 differentially expressed genes (DEGs) were identified after 0.1% and 1% H₂O₂ injection, respectively. These DEGs were mainly involved in the oxidative stress response and immune functions. All these results indicated that oxidative stress could impair both antioxidant defense systems and immune systems. Transcriptome analysis provided valuable information on gene functions associated with the response to oxidative stress in the mud crab.