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Appl Environ Microbiol. 1977 Nov;34(5):518-22.

Double-antibody solid-phase enzyme immunoassay for the detection of staphylococcal enterotoxin A.

Abstract

A simple double-antibody enzyme immunoassay that uses a microtechnique was developed for detecting staphylococcal enterotoxin A in food products. Sample preparation can be completed in less than 15 min. Assay sensitivity ranges from 0.4 ng (20-h test time) to 3.2 ng (1- to 3-h test time) of toxin per ml of prepared sample. Separation and detection of enterotoxin from spiked food products ranged between 72 and 98% of the amount added.

PMID:
337898
[PubMed - indexed for MEDLINE]
PMCID:
PMC242693
Free PMC Article
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