Poly(indole-5-carboxylic acid)/reduced graphene oxide/gold nanoparticles/phage-based electrochemical biosensor for highly specific detection of Yersinia pseudotuberculosis

Mikrochim Acta. 2021 Mar 4;188(4):107. doi: 10.1007/s00604-020-04676-y.

Abstract

Yersinia pseudotuberculosis is an enteric bacterium causing yersiniosis in humans. The existing Yersinia pseudotuberculosis detection methods are time-consuming, requiring a sample pretreatment step, and are unable to discriminate live/dead cells. The current work reports a phage-based electrochemical biosensor for rapid and specific detection of Yersinia pseudotuberculosis. The conductive poly(indole-5-carboxylic acid), reduced graphene oxide, and gold nanoparticles are applied for surface modification of the electrode. They possess ultra-high redox stability and retain 97.7% of current response after performing 50 consecutive cycles of cyclic voltammetry.The specific bacteriophages vB_YepM_ZN18 we isolated from hospital sewage water were immobilized on modified electrodes by Au-NH2 bond between gold nanoparticles and phages. The biosensor fabricated with nanomaterials and phages were utilized to detect Yersinia pseudotuberculosis successfully with detection range of 5.30 × 102 to 1.05 × 107 CFU mL-1, detection limit of 3 CFU mL-1, and assay time of 35 min. Moreover, the biosensor can specifically detect live Yersinia pseudotuberculosis without responding to phage-non-host bacteria and dead Yersinia pseudotuberculosis cells. These results suggest that the proposed biosensor is a promising tool for the rapid and selective detection of Yersinia pseudotuberculosis in food, water, and clinical samples.

Keywords: Poly(indole-5-carboxylic acid); Redox stability; Yersinia pseudotuberculosis detection; vB_YepM_ZN18 phage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Load / instrumentation
  • Bacterial Load / methods*
  • Bacteriophages / chemistry
  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Electrodes
  • Gold / chemistry
  • Graphite / chemistry
  • Indoles / chemistry
  • Limit of Detection
  • Metal Nanoparticles / chemistry
  • Polymers / chemistry
  • Reproducibility of Results
  • Rivers / chemistry
  • Water Pollutants / analysis
  • Yersinia pseudotuberculosis / chemistry
  • Yersinia pseudotuberculosis / isolation & purification*

Substances

  • Indoles
  • Polymers
  • Water Pollutants
  • graphene oxide
  • poly(indole-5-carboxylic acid)
  • Gold
  • Graphite