Ex Vivo Complement Activation on Endothelial Cells: Research and Translational Value

Marie-Sophie Meuleman; Veronique Fremeaux-Bacchi; Lubka T Roumenina; Sophie Chauvet

doi:10.1016/j.molmed.2021.01.008

Ex Vivo Complement Activation on Endothelial Cells: Research and Translational Value

Trends Mol Med. 2021 May;27(5):418-421. doi: 10.1016/j.molmed.2021.01.008. Epub 2021 Feb 26.

Authors

Marie-Sophie Meuleman¹, Veronique Fremeaux-Bacchi¹, Lubka T Roumenina², Sophie Chauvet³

Affiliations

¹ Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université de Paris, F-75006 Paris, France.
² Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université de Paris, F-75006 Paris, France. Electronic address: lubka.roumenina@sorbonne-universite.fr.
³ Centre de Recherche des Cordeliers, INSERM, Sorbonne Université, Université de Paris, F-75006 Paris, France. Electronic address: Sophie.chauvet@aphp.fr.

PMID: 33648869
DOI: 10.1016/j.molmed.2021.01.008

Abstract

The spectrum of human diseases with complement contribution is ever increasing. Tools to study the complement contribution and the potential interest of novel complement inhibitors in clinical practice are lacking. Here we discuss a functional ex vivo assay to monitor complement activation on endothelial cells, which can answer to this need.

Keywords: complement; diagnostics; endothelial cells; kidney injury.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Complement Activation / physiology*
Complement Inactivating Agents
Complement System Proteins* / immunology
Complement System Proteins* / physiology
Endothelial Cells* / immunology
Endothelial Cells* / physiology
Humans
Immunity, Innate

Substances

Complement Inactivating Agents
Complement System Proteins