Neurocysticercosis (NCC) is an important but neglected tropical infectious disease, which is recently recognized as a global problem due to its potentiality for human-to-human transmission beyond tropics. The laboratory diagnosis of NCC is considered useful to confirm clinical and radiological diagnosis. However there is a lack of indigenous diagnostic method particularly in the tropical developing countries. Present study aimed to develop and evaluate indigenously developed anti-cysticercus IgG-ELISAs for possible diagnosis of NCC among patients presenting with seizures. Three indigenous antibody detection assays were developed employing three different antigenic preparations from T. solium metacestode larvae (viz., TsM-CF, TsM-CW and TsM-PS). The overall test results showed varying levels of IgG titers in response to the three antigenic preparations as compared with the standard commercially procured antibody-ELISA. Total soluble protein extract of protoscoleces or TsM-PS-Ag employed in the indigenously developed IgG ELISA is recommended to be used as a routine screening test for a confirmatory diagnosis of NCC and other forms of cysticercosis in humans.