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J Parasitol. 1988 Feb;74(1):111-5.

In vitro cultivation of a Babesia isolated from a white-tailed deer (Odocoileus virginianus).

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  • 1Department of Veterinary Microbiology and Parasitology, Texas A&M University, College Station 77843.


Pyriforms and ring forms of Babesia odocoilei were detected in thin blood smears obtained from a white-tailed deer killed by a hunter in Anderson County, Texas. Erythrocytes from the deer were cultured and the parasites maintained through 8 serial subcultures during 1 mo. The parasite was successfully established in culture using Medium 199 supplemented with either 20% deer serum or 40% normal adult bovine serum. The highest parasitemia observed was 30% and more than 4 parasites per erythrocyte were often observed. Cultured B. odocoilei remained infective for a susceptible white-tailed deer.

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