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Comp Biochem Physiol B. 1988;89(2):317-21.

In vivo and solid state 13C nuclear magnetic resonance studies of tyrosine metabolism during insect cuticle formation.

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  • 1Department of Chemistry, Texas A&M University, College Station 77843.


1. Tyrosine metabolism during pupation can be followed in living Heliothis virescens larvae using nuclear magnetic resonance spectroscopy. 2. Loss of 13C signals from a label at the C-3 position of tyrosine during pupation indicates uptake of tyrosine into solid cuticle. 3. Solids 13C NMR spectroscopy of cuticle formed by insects injected with 3-13C labelled tyrosine indicates that little change in chemical shift occurs during cuticle hardening, indicating that the side chain is probably not involved in protein cross-linking.

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