Comparison of human peripheral blood dendritic cell activation by four fucoidans

Int J Biol Macromol. 2021 Mar 31:174:477-484. doi: 10.1016/j.ijbiomac.2021.01.155. Epub 2021 Jan 26.

Abstract

Brown seaweed is an important source of fucoidan, which displays immunomodulatory effects by activating various immune cells. However, these effects of fucoidans from various sources of brown seaweed have not yet been explored in human blood dendritic cells. We studied fucoidans extracted from Ecklonia cava, Macrocystis pyrifera, Undaria pinnatifida, and Fucus vesiculosus for their effects on human monocyte-derived dendritic cells (MODC) and human peripheral blood DC (PBDC) activation. Ecklonia cava fucoidan (ECF) strongly upregulated co-stimulatory molecules, major histocompatibility complex class I and II, and the production of proinflammatory cytokines in MODCs and PBDCs compared to those by the other three fucoidans. Moreover, ECF elicited the strongest effect in the induction of syngeneic T cell proliferation and IFN-γ production compared to those of other fucoidans. These results suggest that ECF could be a suitable candidate molecule for enhancing immune activation in humans compared to that with the other three fucoidans.

Keywords: Adjuvant; Fucoidans; Helper T cells; Human peripheral blood dendritic cells; Immunity.

Publication types

  • Comparative Study

MeSH terms

  • Cells, Cultured
  • Cytokines / metabolism
  • Dendritic Cells / cytology
  • Dendritic Cells / drug effects
  • Dendritic Cells / immunology*
  • Fucus / chemistry
  • Gene Expression Regulation / drug effects
  • Histocompatibility Antigens Class I / metabolism
  • Histocompatibility Antigens Class II / metabolism
  • Humans
  • Interferon-gamma / metabolism
  • Leukocytes, Mononuclear / cytology*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Macrocystis / chemistry
  • Monocytes / cytology*
  • Monocytes / drug effects
  • Monocytes / immunology
  • Phaeophyceae / chemistry
  • Phaeophyceae / classification*
  • Polysaccharides / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Seaweed
  • T-Lymphocytes / metabolism
  • Undaria / chemistry
  • Up-Regulation

Substances

  • Cytokines
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • IFNG protein, human
  • Polysaccharides
  • Interferon-gamma
  • fucoidan