Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

Moritoshi Sato; Yoshio Umezawa

doi:10.1007/978-1-0716-1091-6_5

Cell-Based Biosensor to Visualize Nitric Oxide Release from Living Cells for Toxicity Assessment

Methods Mol Biol. 2021:2240:57-64. doi: 10.1007/978-1-0716-1091-6_5.

Authors

Moritoshi Sato¹, Yoshio Umezawa²

Affiliations

¹ Graduate School of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo, Japan. cmsato@mail.ecc.u-tokyo.ac.jp.
² Department of Chemistry, School of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo, Japan.

PMID: 33423226
DOI: 10.1007/978-1-0716-1091-6_5

Abstract

Fluorescence imaging provides a powerful technique to observe biomolecular dynamics in living systems, if fluorescent biosensors for the relevant biomolecules become available. Here, we describe a highly sensitive, cell-based biosensor to visualize nitric oxide (NO) released from living cells. Nitric oxide (NO) is a gaseous molecule that is involved in a broad range of physiological and toxicological processes in cardiovascular and central nervous systems, etc. This chapter describes how to make optical measurements of NO release from living cells using the cell-based fluorescent biosensor.

Keywords: Cell-based biosensor; Fluorescence resonance energy transfer (FRET ); Fluorescent biosensor; Live cell imaging; Nitric oxide (NO).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biosensing Techniques / methods*
Cell Line
Cells, Cultured
Endothelial Cells / metabolism
Endothelium, Vascular / cytology
Endothelium, Vascular / metabolism
Fluorescence Resonance Energy Transfer / methods*
Nitric Oxide / metabolism*
Toxicity Tests / methods*

Substances

Nitric Oxide