The bacterial community structure and N-cycling gene abundance in response to dam construction in a riparian zone

Dam construction has significantly altered riparian hydrological regime and environmental conditions in the reservoir region, yet knowledge concerning how bacterial community and N-cycling genes respond to these changes remains limited. In this study, we investigated the bacterial community composition, network structure and N-cycling genes in the water level fluctuation zones (WLFZs) of the Three Gorges Reservoir (TGR). Here, samples collected from five different water levels were divided into three groups: waterward sediments, interface sediments, and landward soils. Our results show that higher contents of NO₂^--N, SOC, DOC, NH₄⁺-N, and TP were characterized in waterward and interface sediments whereas higherNO₃^--N content was observed in landward soils. The α-diversity of bacterial community decreased gradually from waterward sediments to landward soils. Compared with waterward sediments and landward soils, the interface sediments showed a unique bacterial community pattern with diverse primary producers as well as N-cycling microbes. The interface sediments also had a much more complex co-occurrence network and a higher possible community stability. Among all of N-cycling genes, higher abundances of nrfA and AOA amoA genes were observed in interface sediments. The dissimilarity in bacterial community composition and N-cycling gene abundance was mainly driven by water-level. Moreover, random forest model revealed that AOA amoA and nirS genes were the most sensitive indicators in response to water level fluctuations. Overall, this study suggests distinct abundance, diversity, and network structure of microbes in riparian sediments and soils across the gradient of water levels and enhances our understanding with respect to comprehensive effects of dam construction on nitrogen cycle.