Format

Send to:

Choose Destination
See comment in PubMed Commons below
J Clin Invest. 1988 Feb;81(2):620-3.

Peritubular cells are the site of erythropoietin synthesis in the murine hypoxic kidney.

Author information

  • 1Institut National de la Santé et de la Recherche Medicale (INSERM) U152, CNRS UA 628, Hôpital Cochin, Paris, France.

Abstract

Erythropoietin (Epo)-producing cells were identified in the murine hypoxic kidney by in situ hybridization. Profound anemia was induced in order to greatly increase Epo production. This resulted in high levels of Epo mRNA in the kidney. 35S-labeled DNA fragments of the murine Epo gene were used as probes for in situ hybridization. Control experiments conducted in parallel included kidneys of nonanemic mice, RNase-treated hypoxic kidney sections, and 35S-labeled non-Epo-related DNA. The Epo probe gave a specific hybridization signal in the hypoxic kidney in the cortex and to a lesser extent in the outer medulla. Glomerular and tubular cells were not labeled. All positive cells were identified as peritubular cells. Using immunofluorescence, we showed that cells with the same topography contained Factor VIII-related antigen. These data demonstrated that peritubular cells, most likely endothelial cells, constitute the major site of Epo production in the murine hypoxic kidney.

PMID:
3339134
[PubMed - indexed for MEDLINE]
PMCID:
PMC329613
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Journal of Clinical Investigation Icon for PubMed Central
    Loading ...
    Write to the Help Desk