Antiproliferative effect and autophagy induction of curcumin derivative ZYX02-Na on the human lung cancer cells A549

J Biochem Mol Toxicol. 2020 Dec;34(12):e22592. doi: 10.1002/jbt.22592. Epub 2020 Jul 24.

Abstract

At present, a large number of curcumin derivatives had been produced and identified aiming to replace the curcumin in view of its low bioavailability and stability. Here, a novel curcumin derivative ZYX02-Na was first used to reduce the cell viability of human non-small cell lung cells A549, which was confirmed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Flow cytometry and Western blot analysis showed that ZYX02-Na could lead to cell cycle arrest in G0/G1 phase, which demonstrated that ZYX02-Na inhibited the proliferation of A549 cells. Furthermore, the AMPK/mTOR/4E-BP1 signaling pathway was activated in ZYX02-Na-treated A549 cells. Besides, wounding healing and transwell experiments showed that ZYX02-Na could also inhibited the migration ability of A549 cells. Moreover, we also found that ZYX02-Na could induce autophagy of A549 cells by acridine orange staining, GFP-LC3 subcellular localization observation and Western blotting analysis, respectively. In short, our current studies indicated that ZYX02-Na possessed the antiproliferation effect and autophagy induction on A549 cells, while in vivo anticancer study of ZYX02-Na needs to be done in future.

Keywords: A549; autophagy; cell proliferation; curcumin derivative.

MeSH terms

  • A549 Cells
  • Antineoplastic Agents / pharmacology*
  • Autophagy / drug effects*
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • Humans

Substances

  • Antineoplastic Agents