Send to

Choose Destination
See comment in PubMed Commons below
Proc Natl Acad Sci U S A. 1987 Nov;84(22):8035-9.

Isolation, DNA sequence, and regulation of a meiosis-specific eukaryotic recombination gene.

Author information

  • 1Department of Molecular Genetics and Cell Biology, University of Chicago, IL 60637.


The SPO11 gene, required for meiotic recombination in Saccharomyces cerevisiae, has been cloned by direct selection for complementation of the spo11-1 phenotype: lack of meiotic recombination and low spore viability. DNA sequencing indicates that the gene encodes a 398-amino acid protein having a predicted molecular mass of 45.3 kDa. There is no significant similarity between the SPO11 protein and other protein sequences, including those from genes known to be involved in DNA recombination or repair. Strains bearing a disruption allele are viable, indicating that SPO11 is dispensable for mitotic growth. RNA analyses demonstrate that SPO11 produces a 1.5-kilobase transcript that is developmentally regulated and expressed early in the sporulation process.

[PubMed - indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk