Electro-assisted autohydrogenotrophic reduction of perchlorate and microbial community in a dual-chamber biofilm-electrode reactor

The electro-assisted autohydrogenotrophic reduction of perchlorate (ClO₄^-) was investigated in a dual-chamber biofilm-electrode reactor (BER), in which the microbial community was inoculated from natural sediments. To avoid the effect of extreme pH and direct electron transfer on perchlorate reduction, a novel cathode configuration was designed. The pH of the cathode compartment was successfully controlled in the range of 7.2-8.4 during whole experiment. The effective biological autohydrogenotrophic reduction of perchlorate was achieved using hydrogen generated in-situ on the electrode surface, and the removal rate of 10 mg L^-1 perchlorate reached 98.16% at HRT of 48 h. The highest perchlorate removal flux reached to 1498.420 mg m^-2·d^-1 with a 0.410 kW·h g-perchlorate^-1 energy consumption. The microbial community evolution in the BER was determined by high-throughput sequencing and the results indicated that the Firmicutes and Bacteroidetes were dominant at phylum level when perchlorate concentration was 10 mg L^-1 or lower. And the Proteobacteria became ascendant at the perchlorate concentration of 20 mg L^-1. The functional populations for perchlorate reduction were successfully enriched including Nitrosomonas (30%), Thermomonas (9%), Comamonas (8%) and Hydrogenophaga (3%). Meanwhile, the proportion of functional population in biofilm linked to perchlorate concentration. With the increase of influent perchlorate concentration, the perchlorate-reducing bacteria (PRB) were enriched successfully and became ascendant.